rat stem cell factor  (R&D Systems)

Journal: Neurobiology of Stress

Article Title: In vitro modeling of the neurobiological effects of glucocorticoids: A review

doi: 10.1016/j.ynstr.2023.100530

Figure Lengend Snippet: In vitro studies examining the role of glucocorticoids in central nervous system cell lines.

Article Snippet: ( ) , Sprague-Dawley rat primary cortical neural stem cell cultures [E15] , dexamethasone , 1 μM , 48 h , DEX exposure led to a genome wide hypomethylation associated with a decrease in Dnmt3a and an increase in Dkk1 via an increase in Tet3 expression..

Techniques: In Vitro, Concentration Assay, Expressing, Diagnostic Assay, Activity Assay, Methylation, Genome Wide, RNA Expression, DNA Methylation Assay

Journal: bioRxiv

Article Title: Developmental HCN channelopathy results in decreased neural progenitor proliferation and microcephaly in mice

doi: 10.1101/2021.04.24.441237

Figure Lengend Snippet: (A-C) Cortical sections of E12.5 control and EMX1-HCN-DN mutants stained for (A) radial glia cells (Pax6, red), (B) apoptotic (cleaved Caspase-3; cCas3, red), and (C) proliferating cells (Ki67, red), eGFP is depicted in green, DAPI in blue. (D) Quantification of cCas3-positive area in percentage of total analyzed area (unpaired t-test). (E) Quantification of Ki67-positive cells in percentage of DAPI-positive cells (unpaired t-test). (F) Images representative of Ki67 (red) staining of the VZ/SVZ and the sensorimotor cortex in P0 control mice and EMX1-HCN-DN mutants, DAPI is shown in blue. (G, H) Quantification of Ki67 expression (unpaired t-test). (I, J) Analysis of Ki67 mRNA level (I) and BrdU incorporation (J) in rat NSCs treated with ZD7288 (10 µM and 30 µM; Ki67: one–way ANOVA with Tukey’s post-hoc; BrdU: Kruskal-Wallis with post-hoc Dunn’s test). (K) Two-dimensional, tSNE projection of scRNA-seq data from vehicle-treated control (circles, 281 cells), and ZD7288-treated (30 µM, square, 190 cells) rat NSCs. The cell-cycle phase of each cell, which was determined from its gene expression profile, is indicated by the legend (lower left). The inset shows the relative distribution of cell-cycle phases in the vehicle-treated (control) and ZD7288 -treated NSC groups, which were significantly different from each other (p < 0.0001, Chi-square). (L) tSNE projection of the same NSCs as in (K) after regressing out the variance explainable by cell-cycle gene transcripts. Scale bars 100 µm; Data are presented as mean ± s.e.m; n is given in parentheses; p values are above asterisks; Images in (A, B, C) are representative of 5/4 animals, (F) are representative examples of (G, H)

Article Snippet: Rat NSCs (NSC001, R&D systems, P#0-1) were plated on 35-mm dishes (μ-Dish 35mm, high Grid-500 Glass Bottom, Ibidi), and cultured according to the manufacturer’s protocol.

Techniques: Control, Staining, Expressing, BrdU Incorporation Assay, Gene Expression